The tweecer is just a tuner which plugs into the factory computer and allows you to modify the factory tune with your laptop:
The TwEECer -- EEC-IV user programmable module
I pretty much left my timing tables the same, just adding a few degrees to take advantage of the higher octane (as you would for 91), and changed the injector slopes to trick the computer into thinking it was still running gasoline. I would think nearly any tuner would allow you to do that, I just happen to own a tweecer.
I haven't found my gas mileage to take as significant of a nose dive as the energy content would suggest. As I said above, the ethanol promotes a more complete burn to improve efficiency, and of course has a higher octane allowing for higher CRs to recover efficiency. On gasoline, I was usually around 21.something on my normal drive and on E85, I found I was around 19.5-20mpg. I guess that is a change of somewhere between 5-10%, but I must admit I am at WOT more while running E85.
As for mixtures... I saw a technical paper (SAE I think) once which looked at the gas mileage of E10, E20 and E30 mixtures on many different cars. The conclusion was that you generally don't loose and gas mileage on mixtures up to 20%, which they said was due to the more complete burn which the ethanol promotes. Interestingly, some cars gained a little mileage on these mixtures, even to 30%. So it kinda pisses me off when I see people shouting their mouth off about loosing gas mileage on the 10% ethanol gas which is standard these days. Clearly ignorant.